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            <h1 id="seo-header">QP (一) 实时荧光定量PCR结果导出</h1>
            
            
              <div class="markdown-body">
                
                <h2 id="第一步-安装LC96"><a href="#第一步-安装LC96" class="headerlink" title="第一步 安装LC96"></a>第一步 安装LC96</h2><p><a target="_blank" rel="noopener" href="https://lifescience.roche.com/global_en/brands/realtime-pcr-overview.html#software">https://lifescience.roche.com/global_en&#x2F;brands&#x2F;realtime-pcr-overview.html#software</a></p>
<p><a target="_blank" rel="noopener" href="https://limour.lanzoui.com/i7Vrtvxfekd"><img src="https://img-cdn.limour.top/blog_wp/2021/10/image-4.png" srcset="https://jscdn.limour.top/gh/Limour-dev/Sakurairo_Vision/load_svg/inload.svg" lazyload></a></p>
<p>密码:ht91</p>
<h2 id="第二步-查看结果"><a href="#第二步-查看结果" class="headerlink" title="第二步 查看结果"></a>第二步 查看结果</h2><ul>
<li>打开 .lc96p 文件</li>
</ul>
<p><a target="_blank" rel="noopener" href="https://img-cdn.limour.top/blog_wp/2021/10/image-5.png"><img src="https://img-cdn.limour.top/blog_wp/2021/10/image-5.png" srcset="https://jscdn.limour.top/gh/Limour-dev/Sakurairo_Vision/load_svg/inload.svg" lazyload></a></p>
<ul>
<li>给孔命名 样品名 + 引物名</li>
</ul>
<p><a target="_blank" rel="noopener" href="https://img-cdn.limour.top/blog_wp/2021/10/image-7.png"><img src="https://img-cdn.limour.top/blog_wp/2021/10/image-7.png" srcset="https://jscdn.limour.top/gh/Limour-dev/Sakurairo_Vision/load_svg/inload.svg" lazyload></a></p>
<ul>
<li>重新加载命名完的数据</li>
</ul>
<p><a target="_blank" rel="noopener" href="https://img-cdn.limour.top/blog_wp/2021/10/image-9.png"><img src="https://img-cdn.limour.top/blog_wp/2021/10/image-9.png" srcset="https://jscdn.limour.top/gh/Limour-dev/Sakurairo_Vision/load_svg/inload.svg" lazyload></a></p>
<ul>
<li>看引物溶解曲线<br>单峰表明特异性好，且单峰需出现在80℃之后</li>
</ul>
<p><a target="_blank" rel="noopener" href="https://img-cdn.limour.top/blog_wp/2021/10/image-10.png"><img src="https://img-cdn.limour.top/blog_wp/2021/10/image-10.png" srcset="https://jscdn.limour.top/gh/Limour-dev/Sakurairo_Vision/load_svg/inload.svg" lazyload></a></p>
<p><a target="_blank" rel="noopener" href="https://img-cdn.limour.top/blog_wp/2021/10/image-11.png"><img src="https://img-cdn.limour.top/blog_wp/2021/10/image-11.png" srcset="https://jscdn.limour.top/gh/Limour-dev/Sakurairo_Vision/load_svg/inload.svg" lazyload></a></p>
<p>仅有80℃后单峰，说明还不错</p>
<p><a target="_blank" rel="noopener" href="https://img-cdn.limour.top/blog_wp/2021/10/image-12.png"><img src="https://img-cdn.limour.top/blog_wp/2021/10/image-12.png" srcset="https://jscdn.limour.top/gh/Limour-dev/Sakurairo_Vision/load_svg/inload.svg" lazyload></a></p>
<p>双峰，特异性差</p>
<p><a target="_blank" rel="noopener" href="https://zhuanlan.zhihu.com/p/43129856">答疑专栏丨熔解曲线总是出现多峰？来看看是不是这个原因！</a><br><a target="_blank" rel="noopener" href="http://m.zhienbio.com/display/171326.html">qPCR溶解曲线分析：溶解曲线不好？峰值低？怎么办？</a><br><strong><a target="_blank" rel="noopener" href="https://www.yeasen.com/news/detail/235">干货 qPCR常见问题及解决方案全新迭代，快来收藏~</a></strong></p>
<ul>
<li>查看CT值范围</li>
</ul>
<p><a target="_blank" rel="noopener" href="https://img-cdn.limour.top/blog_wp/2021/10/image-13.png"><img src="https://img-cdn.limour.top/blog_wp/2021/10/image-13.png" srcset="https://jscdn.limour.top/gh/Limour-dev/Sakurairo_Vision/load_svg/inload.svg" lazyload></a></p>
<p>15-28之间比较好，低了表示基因表达高，反之则反；太高太低都误差大</p>
<ul>
<li>查看指数增长期曲线是否平行</li>
</ul>
<p><a target="_blank" rel="noopener" href="https://img-cdn.limour.top/blog_wp/2021/10/image-14.png"><img src="https://img-cdn.limour.top/blog_wp/2021/10/image-14.png" srcset="https://jscdn.limour.top/gh/Limour-dev/Sakurairo_Vision/load_svg/inload.svg" lazyload></a></p>
<p>平行，说明扩增效率一致</p>
<p><a target="_blank" rel="noopener" href="https://img-cdn.limour.top/blog_wp/2021/10/image-15.png"><img src="https://img-cdn.limour.top/blog_wp/2021/10/image-15.png" srcset="https://jscdn.limour.top/gh/Limour-dev/Sakurairo_Vision/load_svg/inload.svg" lazyload></a></p>
<p>不 平行，说明扩增效率差异大</p>
<h2 id="第三步-导出结果"><a href="#第三步-导出结果" class="headerlink" title="第三步 导出结果"></a>第三步 导出结果</h2><ul>
<li>复制选择的孔的结果</li>
</ul>
<p><a target="_blank" rel="noopener" href="https://img-cdn.limour.top/blog_wp/2021/10/image-16.png"><img src="https://img-cdn.limour.top/blog_wp/2021/10/image-16.png" srcset="https://jscdn.limour.top/gh/Limour-dev/Sakurairo_Vision/load_svg/inload.svg" lazyload></a></p>
<ul>
<li>粘贴到Excel</li>
</ul>
<p><a target="_blank" rel="noopener" href="https://img-cdn.limour.top/blog_wp/2021/10/image-17.png"><img src="https://img-cdn.limour.top/blog_wp/2021/10/image-17.png" srcset="https://jscdn.limour.top/gh/Limour-dev/Sakurairo_Vision/load_svg/inload.svg" lazyload></a></p>

                
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