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  133. <h1 id="seo-header">使用Rclone搭配OneDrive迁移大量数据</h1>
  134. <div class="markdown-body">
  135. <p>之前在自己的小机器上分析,现在需要在<a target="_blank" rel="noopener" href="https://occdn.limour.top/2398.html">学校集群</a>进行分析,因此需要在两个没有公网ip且不互联的服务器之间转移大量数据。因此计划使用Rclone,通过OneDrive进行中转。</p>
  136. <h2 id="打包需要转移的数据"><a href="#打包需要转移的数据" class="headerlink" title="打包需要转移的数据"></a>打包需要转移的数据</h2><figure class="highlight r"><table><tr><td class="gutter"><pre><span class="line">1</span><br><span class="line">2</span><br><span class="line">3</span><br><span class="line">4</span><br><span class="line">5</span><br><span class="line">6</span><br><span class="line">7</span><br><span class="line">8</span><br><span class="line">9</span><br><span class="line">10</span><br><span class="line">11</span><br><span class="line">12</span><br><span class="line">13</span><br><span class="line">14</span><br><span class="line">15</span><br><span class="line">16</span><br><span class="line">17</span><br><span class="line">18</span><br><span class="line">19</span><br><span class="line">20</span><br><span class="line">21</span><br><span class="line">22</span><br><span class="line">23</span><br><span class="line">24</span><br><span class="line">25</span><br><span class="line">26</span><br><span class="line">27</span><br><span class="line">28</span><br><span class="line">29</span><br><span class="line">30</span><br><span class="line">31</span><br><span class="line">32</span><br><span class="line">33</span><br><span class="line">34</span><br><span class="line">35</span><br><span class="line">36</span><br><span class="line">37</span><br><span class="line">38</span><br><span class="line">39</span><br><span class="line">40</span><br><span class="line">41</span><br></pre></td><td class="code"><pre><code class="hljs R">data <span class="hljs-operator">&lt;-</span> <span class="hljs-built_in">list</span><span class="hljs-punctuation">(</span><span class="hljs-punctuation">)</span><br>ref_sce <span class="hljs-operator">&lt;-</span> readRDS<span class="hljs-punctuation">(</span><span class="hljs-string">&#x27;~/upload/zl_liu/data/pca.rds&#x27;</span><span class="hljs-punctuation">)</span><br>data<span class="hljs-operator">$</span>zyy_umi <span class="hljs-operator">&lt;-</span> ref_sce<span class="hljs-operator">@</span>assays<span class="hljs-operator">$</span>RNA<span class="hljs-operator">@</span>counts<br>data<span class="hljs-operator">$</span>zyy_meta <span class="hljs-operator">&lt;-</span> ref_sce<span class="hljs-operator">@</span>meta.data<br>ref_sce <span class="hljs-operator">&lt;-</span> readRDS<span class="hljs-punctuation">(</span><span class="hljs-string">&#x27;~/work_st/Prognosis/idea_2/fig3.2/fig6/sce.rds&#x27;</span><span class="hljs-punctuation">)</span><br>data<span class="hljs-operator">$</span>ch_umi <span class="hljs-operator">&lt;-</span> ref_sce<span class="hljs-operator">@</span>assays<span class="hljs-operator">$</span>originalexp<span class="hljs-operator">@</span>counts<br>data<span class="hljs-operator">$</span>ch_meta <span class="hljs-operator">&lt;-</span> ref_sce<span class="hljs-operator">@</span>meta.data<br><span class="hljs-comment"># tp_dir &lt;- list(</span><br><span class="hljs-comment"># SRX6887739 = &#x27;~/work_st/sce/GSE137829/res/SRX6887739/outs/filtered_feature_bc_matrix&#x27;,</span><br><span class="hljs-comment"># SRX6887740 = &#x27;~/work_st/sce/GSE137829/res/SRX6887740/outs/filtered_feature_bc_matrix&#x27;,</span><br><span class="hljs-comment"># SRX6887741 = &#x27;~/work_st/sce/GSE137829/res/SRX6887741/outs/filtered_feature_bc_matrix&#x27;,</span><br><span class="hljs-comment"># SRX6887742 = &#x27;~/work_st/sce/GSE137829/res/SRX6887742/outs/filtered_feature_bc_matrix&#x27;,</span><br><span class="hljs-comment"># SRX8890105 = &#x27;~/work_st/sce/GSE137829/res/SRX8890105/outs/filtered_feature_bc_matrix&#x27;,</span><br><span class="hljs-comment"># SRX8890106 = &#x27;~/work_st/sce/GSE137829/res/SRX8890106/outs/filtered_feature_bc_matrix&#x27;</span><br><span class="hljs-comment"># )</span><br><span class="hljs-comment"># counts &lt;- Seurat::Read10X(data.dir = unlist(tp_dir))</span><br><span class="hljs-comment"># sce &lt;- Seurat::CreateSeuratObject(counts, project = &#x27;GSE137829&#x27;,</span><br><span class="hljs-comment"># min.cells = 3, min.features = 200)</span><br><span class="hljs-comment"># data$GSE137829_umi &lt;- sce@assays$RNA@counts</span><br><span class="hljs-comment"># data$GSE137829_meta &lt;- sce@meta.data</span><br>tp_dir <span class="hljs-operator">&lt;-</span> <span class="hljs-built_in">list</span><span class="hljs-punctuation">(</span><br> P1 <span class="hljs-operator">=</span> <span class="hljs-string">&#x27;~/work/GSE137829/GSM4089151_P1_gene_cell_exprs_table.txt.gz&#x27;</span><span class="hljs-punctuation">,</span><br> P2 <span class="hljs-operator">=</span> <span class="hljs-string">&#x27;~/work/GSE137829/GSM4089152_P2_gene_cell_exprs_table.txt.gz&#x27;</span><span class="hljs-punctuation">,</span><br> P3 <span class="hljs-operator">=</span> <span class="hljs-string">&#x27;~/work/GSE137829/GSM4089153_P3_gene_cell_exprs_table.txt.gz&#x27;</span><span class="hljs-punctuation">,</span><br> P4 <span class="hljs-operator">=</span> <span class="hljs-string">&#x27;~/work/GSE137829/GSM4089154_P4_gene_cell_exprs_table.txt.gz&#x27;</span><span class="hljs-punctuation">,</span><br> P5 <span class="hljs-operator">=</span> <span class="hljs-string">&#x27;~/work/GSE137829/GSM4711414_P5_gene_cell_exprs_table.txt.gz&#x27;</span><span class="hljs-punctuation">,</span><br> P6 <span class="hljs-operator">=</span> <span class="hljs-string">&#x27;~/work/GSE137829/GSM4711415_P6_gene_cell_exprs_table.txt.gz&#x27;</span><br><span class="hljs-punctuation">)</span><br>sce <span class="hljs-operator">&lt;-</span> <span class="hljs-built_in">list</span><span class="hljs-punctuation">(</span><span class="hljs-punctuation">)</span><br><span class="hljs-keyword">for</span> <span class="hljs-punctuation">(</span>i <span class="hljs-keyword">in</span> <span class="hljs-built_in">names</span><span class="hljs-punctuation">(</span>tp_dir<span class="hljs-punctuation">)</span><span class="hljs-punctuation">)</span><span class="hljs-punctuation">&#123;</span><br> tmp <span class="hljs-operator">&lt;-</span> read.table<span class="hljs-punctuation">(</span>gzfile<span class="hljs-punctuation">(</span>tp_dir<span class="hljs-punctuation">[[</span>i<span class="hljs-punctuation">]</span><span class="hljs-punctuation">]</span><span class="hljs-punctuation">)</span><span class="hljs-punctuation">,</span> header <span class="hljs-operator">=</span> <span class="hljs-built_in">T</span><span class="hljs-punctuation">)</span><br> umi <span class="hljs-operator">&lt;-</span> Matrix<span class="hljs-operator">::</span>as.matrix<span class="hljs-punctuation">(</span>x <span class="hljs-operator">=</span> tmp<span class="hljs-punctuation">[</span><span class="hljs-operator">-</span><span class="hljs-built_in">c</span><span class="hljs-punctuation">(</span><span class="hljs-number">1</span><span class="hljs-punctuation">,</span><span class="hljs-number">2</span><span class="hljs-punctuation">)</span><span class="hljs-punctuation">]</span><span class="hljs-punctuation">)</span><br> umi <span class="hljs-operator">&lt;-</span> Matrix<span class="hljs-operator">::</span>Matrix<span class="hljs-punctuation">(</span>data <span class="hljs-operator">=</span> umi<span class="hljs-punctuation">,</span> sparse <span class="hljs-operator">=</span> <span class="hljs-built_in">T</span><span class="hljs-punctuation">)</span><br> rownames<span class="hljs-punctuation">(</span>umi<span class="hljs-punctuation">)</span> <span class="hljs-operator">&lt;-</span> tmp<span class="hljs-operator">$</span>Symbol<br> sce<span class="hljs-punctuation">[[</span>i<span class="hljs-punctuation">]</span><span class="hljs-punctuation">]</span> <span class="hljs-operator">&lt;-</span> Seurat<span class="hljs-operator">::</span>CreateSeuratObject<span class="hljs-punctuation">(</span>umi<span class="hljs-punctuation">,</span> project <span class="hljs-operator">=</span> i<span class="hljs-punctuation">,</span><br> min.cells <span class="hljs-operator">=</span> <span class="hljs-number">3</span><span class="hljs-punctuation">,</span> min.features <span class="hljs-operator">=</span> <span class="hljs-number">200</span><span class="hljs-punctuation">)</span><br><span class="hljs-punctuation">&#125;</span><br>sce <span class="hljs-operator">&lt;-</span> Reduce<span class="hljs-punctuation">(</span>merge<span class="hljs-punctuation">,</span> sce<span class="hljs-punctuation">)</span><br>data<span class="hljs-operator">$</span>geo_umi <span class="hljs-operator">&lt;-</span> sce<span class="hljs-operator">@</span>assays<span class="hljs-operator">$</span>RNA<span class="hljs-operator">@</span>counts<br>data<span class="hljs-operator">$</span>geo_meta <span class="hljs-operator">&lt;-</span> sce<span class="hljs-operator">@</span>meta.data<br>saveRDS<span class="hljs-punctuation">(</span>data<span class="hljs-punctuation">,</span> <span class="hljs-string">&#x27;22.10.04.rds&#x27;</span><span class="hljs-punctuation">)</span><br></code></pre></td></tr></table></figure>
  137. <h2 id="Rclone挂载OneDrive"><a href="#Rclone挂载OneDrive" class="headerlink" title="Rclone挂载OneDrive"></a>Rclone挂载OneDrive</h2><ul>
  138. <li>conda activate jupyter</li>
  139. <li>conda install -c conda-forge rclone -y</li>
  140. </ul>
  141. <p>在两台服务器上<a target="_blank" rel="noopener" href="https://occdn.limour.top/2083.html">挂载同一个OneDrive</a>,第二台可以<a target="_blank" rel="noopener" href="https://occdn.limour.top/2088.html">直接使用第一台的配置</a></p>
  142. <h2 id="Rclone上传下载数据"><a href="#Rclone上传下载数据" class="headerlink" title="Rclone上传下载数据"></a>Rclone上传下载数据</h2><ul>
  143. <li>rclone copy –ignore-existing –progress –ignore-errors –transfers&#x3D;1 .&#x2F;22.10.04.rds onedrive:tmp</li>
  144. <li>rclone ls onedrive:tmp</li>
  145. <li>rclone copy –ignore-existing –progress –ignore-errors –transfers&#x3D;1 onedrive:tmp&#x2F;22.10.04.rds .</li>
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