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          安装CellTypist并配置环境，加载所需的包，加载数据，将Seurat对象转换为scanpy对象，对每个细胞进行观察，对基因矩阵进行注释，组装AnnData对象，进行预测，并将预测结果添加到Seurat对象中。
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          【迁移】二代测序数据处理之数据格式说明
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          FASTA(.fa) 储存参考数据集 从零开始完整学习全基因组测序（WGS）数据分析：第2节 FASTA和FASTQ  基本单元 序列所表示的基因名：&gt;ENSMUSG00000020122ENSMUST00000138518，后可接空格表示注释前缀 具体序列信息：CCCTCCTATCATGC……GGGCCCACCTGTTCTCTGGT 基因名独占一行，序列信息为基因名后一行至下一个 &gt;
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              2022-01-17
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              <a href="/tags/ngs/">#NGS</a>
            
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          这段Python代码实现了使用后缀表达式解方程的功能。它包括了一个栈类（Stack）和一个多项式类（Polynomial）。通过中缀表达式转后缀表达式的方式处理方程，最终实现了解一元一次方程的功能。代码包含了多个方法，如多项式的加法、减法、乘法、除法等操作。在解方程时，它先进行表达式的简化和转换，然后输出方程的解或者判断方程是否是一元一次方程。
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              2020-12-12
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              <a href="/tags/%E6%8E%A2%E7%B4%A2/">#探索</a>
            
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          开启 SSH 连接  boot 目录下新建 ssh 的空文件 boot 目录下新建 wpa_supplicant.conf  1234567891011121314151617country=CNctrl_interface=DIR=/var/run/wpa_supplicant GROUP=netdevupdate_config=1 network=&#123;ssid=&quot;Limour
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              2020-03-31
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              <a href="/tags/raspberrypi/">#raspberrypi</a>
            
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