conda create -n music -c conda-forge r-base=4.1.3 -y conda activate music conda install -c conda-forge r-tidyverse=1.3.1 -y conda install -c conda-forge r-irkernel -y conda install -c conda

conda create -n ggsurvplot -c conda-forge r-base=4.1.3 -y conda activate ggsurvplot conda install -c conda-forge r-tidyverse=1.3.1 -y conda install -c conda-forge r-irkernel -y conda instal

计算123456789scv.pp.moments(adata, n_pcs=30, n_neighbors=30)scv.tl.recover_dynamics(adata, n_jobs=8)scv.tl.velocity(adata, mode='dynamical')scv.tl.velocity_graph(adata, n_jobs=8)adata.write(&#

https://www.bilibili.com/read/cv7751387 https://www.bilibili.com/read/cv7760100 https://www.bilibili.com/read/cv7764833 https://www.jianshu.com/p/1e7acde1a318 第一步 导入模块1234567import scvelo as scvimport

1234library(Seurat)library(tidyverse)library(stringr)sce <- readRDS("~/upload/zl_liu/data/pca.rds") 123456789101112131415161718192021222324f_scVelo_group_by <- function(df, groupN)

第一步 下载镜像https://www.right.com.cn/forum/thread-4053752-1-1.html 第二步 解压得到img文件第三步 建立虚拟机第四步 使用kali虚拟机挂载第三步的空白磁盘修改启动顺序：https://jingyan.baidu.com/article/fa4125ac0da02c28ac709280.html 第五步 共享文件到kalipython.e

01.道可道也，非恒道也。名可名也，非恒名也。无名万物之始也，有名万物之母也。故：恒无欲也，以观其眇；恒有欲也，以观其徼。两者同出，异名同谓。玄之又玄，众妙之门。 02.天下皆知美之为美，恶矣，皆知善之为善，斯不善矣。有无之相生也，难易之相成也，长短之相形也，高下之相盈也，音声之相和也，先后之相随也。是以圣人居无为之事，行不言之教。万物作而弗始也，为而弗恃也，成功而弗居也，夫唯弗居，是以弗去。 0

123456789101112conda activate velocyto#!/bin/bashdb=/opt/cellranger/refdata-gex-GRCh38-2020-Awork=/home/jovyan/upload/zl_liu/data/data/resrmsk_gtf=/opt/cellranger/GRCh38_rmsk.gtf # 从genome.ucsc.edu下载

重命名R1、R2data├── hPB003│ ├── hPB003_S1_L001_R1_001.fastq.gz│ └── hPB003_S1_L001_R2_001.fastq.gz├── hPB004│ ├── hPB004_S1_L001_R1_001.fastq.gz│ └── hPB004_S1_L001_R2_001.fastq.gz├── hPB005│ ├── hPB005_S

cellrangerhttps://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/installation https://www.jianshu.com/p/1e7acde1a318 https://mp.weixin.qq.com/s/TJsdj7qesZGlvmEkNLAy0A GR