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            <h1 id="seo-header">通过conda安装纯净环境的CellChat</h1>
            
            
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<li><a target="_blank" rel="noopener" href="https://anaconda.org/conda-forge/r-base">https://anaconda.org/conda-forge/r-base</a></li>
<li>conda create -n cellchat -c conda-forge r-base&#x3D;4.1.2 -y</li>
<li>conda activate cellchat</li>
<li>conda install -c conda-forge r-seurat&#x3D;4.1.0 -y</li>
<li>conda install -c conda-forge r-irkernel&#x3D;1.3 -y</li>
<li>conda install -c conda-forge r-biocmanager&#x3D;1.30.16 -y</li>
<li>conda install -c conda-forge r-devtools&#x3D;2.4.3 -y</li>
<li>conda install -c conda-forge r-circlize&#x3D;0.4.14 -y</li>
<li>conda install -c conda-forge umap-learn&#x3D;0.5.3 -y</li>
<li>conda install -c bioconda bioconductor-biobase&#x3D;2.54.0 -y</li>
<li>wget <a target="_blank" rel="noopener" href="https://github.com/sqjin/CellChat/archive/refs/heads/master.zip">https://github.com/sqjin/CellChat/archive/refs/heads/master.zip</a> -O CellChat-master.zip</li>
<li><strong>移除CellChat-master.zip里src目录下的*.so和*.o文件</strong></li>
<li>unzip CellChat-master.zip</li>
<li>rm CellChat-master&#x2F;src&#x2F;*.so</li>
<li>rm CellChat-master&#x2F;src&#x2F;*.o</li>
<li>wget <a target="_blank" rel="noopener" href="https://github.com/jokergoo/ComplexHeatmap/archive/refs/heads/master.zip">https://github.com/jokergoo/ComplexHeatmap/archive/refs/heads/master.zip</a> -O ComplexHeatmap-master.zip</li>
<li>conda install -c conda-forge r-svglite&#x3D;2.1.0 -y</li>
<li>install.packages(‘NMF’)</li>
<li>devtools::install_local(‘ComplexHeatmap-master.zip’)</li>
<li>devtools::install_local(‘CellChat-master’)</li>
<li>IRkernel::installspec(name&#x3D;’cellchat’, displayname&#x3D;’r-cellchat’)</li>
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<figure class="highlight r"><table><tr><td class="gutter"><pre><span class="line">1</span><br><span class="line">2</span><br><span class="line">3</span><br><span class="line">4</span><br></pre></td><td class="code"><pre><code class="hljs r">Sys.setenv<span class="hljs-punctuation">(</span>RETICULATE_PYTHON <span class="hljs-operator">=</span> <span class="hljs-string">&quot;/opt/conda/envs/cellchat/bin/python3.7&quot;</span><span class="hljs-punctuation">)</span><br>library<span class="hljs-punctuation">(</span><span class="hljs-string">&#x27;reticulate&#x27;</span><span class="hljs-punctuation">)</span><br>use_condaenv<span class="hljs-punctuation">(</span><span class="hljs-string">&quot;cellchat&quot;</span><span class="hljs-punctuation">)</span><br>py_config<span class="hljs-punctuation">(</span><span class="hljs-punctuation">)</span><br></code></pre></td></tr></table></figure>
                
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