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- 第一步 明确CSOmap所需的数据 先读readme,里面描述了输入数据的格式 label.txt:细胞标注表,格式如图 LR_pairs.txt:受体配体表,格式如图,用CSOmap自带的也行 TPM.txt:TPM标注化的表达矩阵,格式如图,第一行得用\t开头 第二步 导出CSOmap所需的数据123456789101112131415161718192021222324
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- 第一步 下载所需的参考数据集 确定目标:Human Multiple Cortical Areas SMART-seq 下载:Gene expression matrix 和 Table of cell metadata 123mkdir Human_Multiple_Cortical_Areas_SMART-seq && cd Human_Multiple_Cortical_A
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- 第一步 导入程辑包123456library(Matrix)library(Seurat)library(plyr)library(dplyr)library(patchwork)library(purrr) 第二步 配置数据路径工作路径1234567# 配置数据和mark基因表的路径root_path = "~/zlliu/R_data/hBLA"# 配置结果保存路径out
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- 第一步 安装 Cairo包 第二步 .Rprofile 文件添加以下内容 1options(bitmapType='cairo') 第三步 ~/.local/share/jupyter/kernels/ir/kernel.json 12345{ "argv": ["/home/rqzhang/zlliu/dev/R/R_s/l
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- 安装BiocManager:install.packages("BiocManager", repos="http://mirrors.tuna.tsinghua.edu.cn/CRAN/" ) 为BiocManager添加镜像:options(BioC_mirror="https://mirrors.tuna.tsinghua.edu.cn/b
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