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  150. <h2 class="index-header">
  151. <a href="/-shell-jie-xi-json" target="_self">
  152. 【迁移】核酸检测完成情况提醒bot:shell解析json
  153. </a>
  154. </h2>
  155. <a class="index-excerpt " href="/-shell-jie-xi-json" target="_self">
  156. <div>
  157. 之前做了个核酸检测完成情况提醒bot,来给班群三天两次进行提醒。运行了一段时间后,发现微信每15天就会踢人下线,有点恶心。因此代码里的toUserName就不能再写死了,得通过api每天获取。这样就被踢了就只要重新登录一下,不用再改toUserName了。 获取UserName sudo apt install jq nano 04.sh chmod +x 04.sh 123456789101
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  169. <a href="/tags/shell/">#shell</a>
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  177. <a href="/-chuan-chu-shen-jing-xi-tong-yao-wu-zhi-dan-jian-shou-ti" target="_self">
  178. <img src="https://api.limour.top/randomImg?d=2022-10-07 17:03:17" srcset="https://jscdn.limour.top/gh/Limour-dev/Sakurairo_Vision/load_svg/inload.svg" lazyload alt="【迁移】药理一:传出神经系统药物之胆碱受体">
  179. </a>
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  182. <h2 class="index-header">
  183. <a href="/-chuan-chu-shen-jing-xi-tong-yao-wu-zhi-dan-jian-shou-ti" target="_self">
  184. 【迁移】药理一:传出神经系统药物之胆碱受体
  185. </a>
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  187. <a class="index-excerpt " href="/-chuan-chu-shen-jing-xi-tong-yao-wu-zhi-dan-jian-shou-ti" target="_self">
  188. <div>
  189. 传出神经分植物神经和运动神经,前者支配心肌、平滑肌和腺体等,后者支配骨骼肌。植物神经分交感和副交感(副交感节前纤维常在迷走神经内走行),交感兴奋时效应器表现为应急时的状态,副交感兴奋时效应器表现为睡眠时的状态。 ACh、AChE相关药 拟胆碱药分两类,兴奋受体抑制酶 匹罗卡品作用眼,外用治疗青光眼 阿托交替芸香碱,防止虹膜晶状粘 贝胆碱、腹气胀,兴奋泌尿和胃肠 新斯地明抗酯酶,主治重症肌无力 术
  190. </div>
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  194. <i class="iconfont icon-date"></i>
  195. <time datetime="2022-10-07 17:03" pubdate>
  196. 2022-10-07
  197. </time>
  198. </div>
  199. <div class="post-meta">
  200. <i class="iconfont icon-tags"></i>
  201. <a href="/tags/%E8%8D%AF%E7%90%86/">#药理</a>
  202. </div>
  203. </div>
  204. </article>
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  206. <div class="row mx-auto index-card">
  207. <div class="col-12 col-md-4 m-auto index-img">
  208. <a href="/-chuan-chu-shen-jing-xi-tong-yao-wu-zhi-shen-shang-xian-shou-ti" target="_self">
  209. <img src="https://api.limour.top/randomImg?d=2022-10-07 16:50:50" srcset="https://jscdn.limour.top/gh/Limour-dev/Sakurairo_Vision/load_svg/inload.svg" lazyload alt="【迁移】药理二:传出神经系统药物之肾上腺受体">
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  213. <h2 class="index-header">
  214. <a href="/-chuan-chu-shen-jing-xi-tong-yao-wu-zhi-shen-shang-xian-shou-ti" target="_self">
  215. 【迁移】药理二:传出神经系统药物之肾上腺受体
  216. </a>
  217. </h2>
  218. <a class="index-excerpt " href="/-chuan-chu-shen-jing-xi-tong-yao-wu-zhi-shen-shang-xian-shou-ti" target="_self">
  219. <div>
  220. 激动药 药物种类 麻黄碱、多巴胺,α β 肾上腺 去甲肾、间羟胺,β 只有异丙肾 肾上腺素 α β 受体兴奋药,肾上腺素是代表 血管收缩血压升,局麻用它延时间 局部止血效明显,过敏休克当首选 心脏兴奋气管扩,哮喘持续它能缓 心跳骤停用三联,应用注意心血管 α 受体被阻断,升压作用能翻转 心衰高压脑硬化,甲亢糖尿都禁用 多巴胺 α β 多巴胺,心脏兴奋血压升 肾脏调节分两种,中低多巴血流增 高浓α
  221. </div>
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  225. <i class="iconfont icon-date"></i>
  226. <time datetime="2022-10-07 16:50" pubdate>
  227. 2022-10-07
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  230. <div class="post-meta">
  231. <i class="iconfont icon-tags"></i>
  232. <a href="/tags/%E8%8D%AF%E7%90%86/">#药理</a>
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  239. <a href="/19-ji-lin-ba-yao-li-di-yi-ci-xiao-ce" target="_self">
  240. <img src="https://api.limour.top/randomImg?d=2022-10-07 16:48:06" srcset="https://jscdn.limour.top/gh/Limour-dev/Sakurairo_Vision/load_svg/inload.svg" lazyload alt="【迁移】19级临八药理第一次小测">
  241. </a>
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  243. <article class="col-12 col-md-8 mx-auto index-info">
  244. <h2 class="index-header">
  245. <a href="/19-ji-lin-ba-yao-li-di-yi-ci-xiao-ce" target="_self">
  246. 【迁移】19级临八药理第一次小测
  247. </a>
  248. </h2>
  249. <a class="index-excerpt " href="/19-ji-lin-ba-yao-li-di-yi-ci-xiao-ce" target="_self">
  250. <div>
  251. 拟胆碱药物是(A) A Physostigmine B Anisodamine C Solifenacin D Scopolamine E Atropine 主要发挥中枢抗胆碱作用的药物是(A) A 东莨菪碱 B 毒扁豆碱 C 山莨菪碱 D 阿托品 E 毛果芸香碱 去甲肾上腺素治疗消化道出血时的给药途径为(A) A 口服 B 肌内注射 C 舌下给药 D 皮下注射 E 静脉滴注 A
  252. </div>
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  256. <i class="iconfont icon-date"></i>
  257. <time datetime="2022-10-07 16:48" pubdate>
  258. 2022-10-07
  259. </time>
  260. </div>
  261. <div class="post-meta">
  262. <i class="iconfont icon-tags"></i>
  263. <a href="/tags/%E8%8D%AF%E7%90%86/">#药理</a>
  264. </div>
  265. </div>
  266. </article>
  267. </div>
  268. <div class="row mx-auto index-card">
  269. <div class="col-12 col-md-4 m-auto index-img">
  270. <a href="/-dao-chu-SingleR-xu-yao-de-shu-ju" target="_self">
  271. <img src="https://api.limour.top/randomImg?d=2022-10-04 17:20:44" srcset="https://jscdn.limour.top/gh/Limour-dev/Sakurairo_Vision/load_svg/inload.svg" lazyload alt="【迁移】导出SingleR需要的数据">
  272. </a>
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  274. <article class="col-12 col-md-8 mx-auto index-info">
  275. <h2 class="index-header">
  276. <a href="/-dao-chu-SingleR-xu-yao-de-shu-ju" target="_self">
  277. 【迁移】导出SingleR需要的数据
  278. </a>
  279. </h2>
  280. <a class="index-excerpt " href="/-dao-chu-SingleR-xu-yao-de-shu-ju" target="_self">
  281. <div>
  282. loom文件使用记录 随着单细胞数据量的增长,计算要求成指数增长,当数据量大于10万个细胞的时候,seurat包分析就显得非常有压力了,因为在实时内存中储存数据就变得非常困难,HDF5数据格式提供了高效的磁盘存储,而不是在内存中存储数据,这就将分析扩展到大规模数据集,甚至可以达到大于100万细胞的级别 ,Linnarson实验室开发了一种基于hdf5的数据结构,loom,可以方便地存储单细胞基因组
  283. </div>
  284. </a>
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  286. <div class="post-meta mr-3">
  287. <i class="iconfont icon-date"></i>
  288. <time datetime="2022-10-04 17:20" pubdate>
  289. 2022-10-04
  290. </time>
  291. </div>
  292. <div class="post-meta">
  293. <i class="iconfont icon-tags"></i>
  294. <a href="/tags/SingleR/">#SingleR</a>
  295. </div>
  296. </div>
  297. </article>
  298. </div>
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  300. <div class="col-12 col-md-4 m-auto index-img">
  301. <a href="/gei-VMware-xu-ni-ji-jia-ying-pan" target="_self">
  302. <img src="https://api.limour.top/randomImg?d=2022-10-02 17:55:18" srcset="https://jscdn.limour.top/gh/Limour-dev/Sakurairo_Vision/load_svg/inload.svg" lazyload alt="【迁移】给VMware虚拟机加硬盘">
  303. </a>
  304. </div>
  305. <article class="col-12 col-md-8 mx-auto index-info">
  306. <h2 class="index-header">
  307. <a href="/gei-VMware-xu-ni-ji-jia-ying-pan" target="_self">
  308. 【迁移】给VMware虚拟机加硬盘
  309. </a>
  310. </h2>
  311. <a class="index-excerpt " href="/gei-VMware-xu-ni-ji-jia-ying-pan" target="_self">
  312. <div>
  313. 买了一台零刻 SER5 Pro 32G作为虚拟机的宿主机,用了一个月感觉还行,准备加装一个铠侠TC10 1T SATA3固态。小心排线接口上有个黑色的东东,先用镊子挑起来再拔。然后虚拟机设置里添加磁盘。 挂载磁盘并设置开机自动mount sudo su fdisk -l #i查看可挂载磁盘 mkfs.ext4 /dev/sdb #格式化为ext4 # apt install util-linux
  314. </div>
  315. </a>
  316. <div class="index-btm post-metas">
  317. <div class="post-meta mr-3">
  318. <i class="iconfont icon-date"></i>
  319. <time datetime="2022-10-02 17:55" pubdate>
  320. 2022-10-02
  321. </time>
  322. </div>
  323. <div class="post-meta">
  324. <i class="iconfont icon-tags"></i>
  325. <a href="/tags/VMware/">#VMware</a>
  326. </div>
  327. </div>
  328. </article>
  329. </div>
  330. <div class="row mx-auto index-card">
  331. <div class="col-12 col-md-4 m-auto index-img">
  332. <a href="/ji-yu-NMF-fen-jie-de-jiang-wei-ju-lei" target="_self">
  333. <img src="https://api.limour.top/randomImg?d=2022-10-02 17:28:30" srcset="https://jscdn.limour.top/gh/Limour-dev/Sakurairo_Vision/load_svg/inload.svg" lazyload alt="【迁移】基于NMF分解的降维聚类">
  334. </a>
  335. </div>
  336. <article class="col-12 col-md-8 mx-auto index-info">
  337. <h2 class="index-header">
  338. <a href="/ji-yu-NMF-fen-jie-de-jiang-wei-ju-lei" target="_self">
  339. 【迁移】基于NMF分解的降维聚类
  340. </a>
  341. </h2>
  342. <a class="index-excerpt " href="/ji-yu-NMF-fen-jie-de-jiang-wei-ju-lei" target="_self">
  343. <div>
  344. 安装补充包 conda activate seurat conda install -c conda-forge r-nmf -y conda install -c conda-forge r-fastica -y 获取非负表达矩阵 使用《使用metacell进行分群聚类》中的数据 1234567891011121314151617sce &lt;- readRDS(&#x27;SRX8890
  345. </div>
  346. </a>
  347. <div class="index-btm post-metas">
  348. <div class="post-meta mr-3">
  349. <i class="iconfont icon-date"></i>
  350. <time datetime="2022-10-02 17:28" pubdate>
  351. 2022-10-02
  352. </time>
  353. </div>
  354. <div class="post-meta">
  355. <i class="iconfont icon-tags"></i>
  356. <a href="/tags/nmf/">#nmf</a>
  357. </div>
  358. </div>
  359. </article>
  360. </div>
  361. <div class="row mx-auto index-card">
  362. <div class="col-12 col-md-4 m-auto index-img">
  363. <a href="/shi-yong-metacell-jin-hang-fen-qun-ju-lei" target="_self">
  364. <img src="https://api.limour.top/randomImg?d=2022-10-01 17:42:22" srcset="https://jscdn.limour.top/gh/Limour-dev/Sakurairo_Vision/load_svg/inload.svg" lazyload alt="【迁移】使用metacell进行分群聚类">
  365. </a>
  366. </div>
  367. <article class="col-12 col-md-8 mx-auto index-info">
  368. <h2 class="index-header">
  369. <a href="/shi-yong-metacell-jin-hang-fen-qun-ju-lei" target="_self">
  370. 【迁移】使用metacell进行分群聚类
  371. </a>
  372. </h2>
  373. <a class="index-excerpt " href="/shi-yong-metacell-jin-hang-fen-qun-ju-lei" target="_self">
  374. <div>
  375. 预处理 12345678910111213141516171819202122232425262728293031f_QC_plot &lt;- function(sce)&#123; options(repr.plot.width = 12, repr.plot.height = 6) print(Seurat::VlnPlot(sce, features = c(&quot;nFe
  376. </div>
  377. </a>
  378. <div class="index-btm post-metas">
  379. <div class="post-meta mr-3">
  380. <i class="iconfont icon-date"></i>
  381. <time datetime="2022-10-01 17:42" pubdate>
  382. 2022-10-01
  383. </time>
  384. </div>
  385. <div class="post-meta">
  386. <i class="iconfont icon-tags"></i>
  387. <a href="/tags/metacell/">#metacell</a>
  388. </div>
  389. </div>
  390. </article>
  391. </div>
  392. <div class="row mx-auto index-card">
  393. <div class="col-12 col-md-4 m-auto index-img">
  394. <a href="/shi-yong-FindTransferAnchors-dui-yang-ben-jin-hang-yu-zhu-shi" target="_self">
  395. <img src="https://api.limour.top/randomImg?d=2022-09-29 17:58:50" srcset="https://jscdn.limour.top/gh/Limour-dev/Sakurairo_Vision/load_svg/inload.svg" lazyload alt="【迁移】使用FindTransferAnchors对样本进行预注释">
  396. </a>
  397. </div>
  398. <article class="col-12 col-md-8 mx-auto index-info">
  399. <h2 class="index-header">
  400. <a href="/shi-yong-FindTransferAnchors-dui-yang-ben-jin-hang-yu-zhu-shi" target="_self">
  401. 【迁移】使用FindTransferAnchors对样本进行预注释
  402. </a>
  403. </h2>
  404. <a class="index-excerpt " href="/shi-yong-FindTransferAnchors-dui-yang-ben-jin-hang-yu-zhu-shi" target="_self">
  405. <div>
  406. 处理参考样本 12345678910111213ref_sce &lt;- readRDS(&#x27;~/upload/zl_liu/data/pca.rds&#x27;)ref_sce &lt;- subset(ref_sce, group == &#x27;CRPC&#x27;)table(ref_sce@meta.data$cell_type_fig3)g2m_genes &lt;- Se
  407. </div>
  408. </a>
  409. <div class="index-btm post-metas">
  410. <div class="post-meta mr-3">
  411. <i class="iconfont icon-date"></i>
  412. <time datetime="2022-09-29 17:58" pubdate>
  413. 2022-09-29
  414. </time>
  415. </div>
  416. <div class="post-meta">
  417. <i class="iconfont icon-tags"></i>
  418. <a href="/tags/FindTransferAnchors/">#FindTransferAnchors</a>
  419. </div>
  420. </div>
  421. </article>
  422. </div>
  423. <div class="row mx-auto index-card">
  424. <div class="col-12 col-md-4 m-auto index-img">
  425. <a href="/shi-yong-SCTransform-biao-zhun-hua" target="_self">
  426. <img src="https://api.limour.top/randomImg?d=2022-09-29 17:32:00" srcset="https://jscdn.limour.top/gh/Limour-dev/Sakurairo_Vision/load_svg/inload.svg" lazyload alt="【迁移】使用SCTransform标准化">
  427. </a>
  428. </div>
  429. <article class="col-12 col-md-8 mx-auto index-info">
  430. <h2 class="index-header">
  431. <a href="/shi-yong-SCTransform-biao-zhun-hua" target="_self">
  432. 【迁移】使用SCTransform标准化
  433. </a>
  434. </h2>
  435. <a class="index-excerpt " href="/shi-yong-SCTransform-biao-zhun-hua" target="_self">
  436. <div>
  437. 安装包 conda create -n seurat -c conda-forge r-seurat=4.1.1 -y conda activate seurat conda install -c conda-forge r-tidyverse -y conda install -c conda-forge r-irkernel -y Rscript -e “IRkernel::installs
  438. </div>
  439. </a>
  440. <div class="index-btm post-metas">
  441. <div class="post-meta mr-3">
  442. <i class="iconfont icon-date"></i>
  443. <time datetime="2022-09-29 17:32" pubdate>
  444. 2022-09-29
  445. </time>
  446. </div>
  447. <div class="post-meta">
  448. <i class="iconfont icon-tags"></i>
  449. <a href="/tags/SCTransform/">#SCTransform</a>
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