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- <h1 id="seo-header">一些常见图的美化</h1>
-
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- <div class="markdown-body">
-
- <h2 id="火山图"><a href="#火山图" class="headerlink" title="火山图"></a>火山图</h2><ul>
- <li><a target="_blank" rel="noopener" href="https://mp.weixin.qq.com/s/TvAor9voh/_kDnIxsC-U48w">https://mp.weixin.qq.com/s/TvAor9voh\_kDnIxsC-U48w</a></li>
- <li><a target="_blank" rel="noopener" href="https://www.bioconductor.org/packages/release/bioc/vignettes/EnhancedVolcano/inst/doc/EnhancedVolcano.html">https://www.bioconductor.org/packages/release/bioc/vignettes/EnhancedVolcano/inst/doc/EnhancedVolcano.html</a></li>
- </ul>
- <figure class="highlight r"><table><tr><td class="gutter"><pre><span class="line">1</span><br><span class="line">2</span><br><span class="line">3</span><br><span class="line">4</span><br><span class="line">5</span><br><span class="line">6</span><br><span class="line">7</span><br><span class="line">8</span><br></pre></td><td class="code"><pre><code class="hljs r">library<span class="hljs-punctuation">(</span>EnhancedVolcano<span class="hljs-punctuation">)</span><br>data <span class="hljs-operator"><-</span> read.csv<span class="hljs-punctuation">(</span><span class="hljs-string">'findmarkers_all.csv'</span><span class="hljs-punctuation">,</span> row.names <span class="hljs-operator">=</span> <span class="hljs-number">1</span><span class="hljs-punctuation">)</span><br>pdf<span class="hljs-punctuation">(</span><span class="hljs-string">'EnhancedVolcano.pdf'</span><span class="hljs-punctuation">,</span> width <span class="hljs-operator">=</span> <span class="hljs-number">9</span><span class="hljs-punctuation">,</span> height <span class="hljs-operator">=</span> <span class="hljs-number">9</span><span class="hljs-punctuation">)</span><br>EnhancedVolcano<span class="hljs-punctuation">(</span>data<span class="hljs-punctuation">,</span><br> lab <span class="hljs-operator">=</span> rownames<span class="hljs-punctuation">(</span>data<span class="hljs-punctuation">)</span><span class="hljs-punctuation">,</span><br> x <span class="hljs-operator">=</span> <span class="hljs-string">'avg_log2FC'</span><span class="hljs-punctuation">,</span><br> y <span class="hljs-operator">=</span> <span class="hljs-string">'p_val_adj'</span><span class="hljs-punctuation">,</span> pCutoff <span class="hljs-operator">=</span> <span class="hljs-number">1e-10</span><span class="hljs-punctuation">)</span><br>dev.off<span class="hljs-punctuation">(</span><span class="hljs-punctuation">)</span><br></code></pre></td></tr></table></figure>
- <h2 id="和弦图"><a href="#和弦图" class="headerlink" title="和弦图"></a>和弦图</h2><ul>
- <li><a target="_blank" rel="noopener" href="https://blog.csdn.net/weixin/_43528109/article/details/83819728">https://blog.csdn.net/weixin\_43528109/article/details/83819728</a></li>
- <li><a target="_blank" rel="noopener" href="https://my.oschina.net/u/4579431/blog/4347371">https://my.oschina.net/u/4579431/blog/4347371</a></li>
- </ul>
- <figure class="highlight r"><table><tr><td class="gutter"><pre><span class="line">1</span><br><span class="line">2</span><br><span class="line">3</span><br><span class="line">4</span><br><span class="line">5</span><br><span class="line">6</span><br><span class="line">7</span><br><span class="line">8</span><br><span class="line">9</span><br><span class="line">10</span><br><span class="line">11</span><br><span class="line">12</span><br><span class="line">13</span><br><span class="line">14</span><br><span class="line">15</span><br><span class="line">16</span><br><span class="line">17</span><br><span class="line">18</span><br><span class="line">19</span><br><span class="line">20</span><br><span class="line">21</span><br><span class="line">22</span><br><span class="line">23</span><br><span class="line">24</span><br><span class="line">25</span><br><span class="line">26</span><br><span class="line">27</span><br><span class="line">28</span><br><span class="line">29</span><br><span class="line">30</span><br><span class="line">31</span><br><span class="line">32</span><br><span class="line">33</span><br><span class="line">34</span><br><span class="line">35</span><br><span class="line">36</span><br><span class="line">37</span><br><span class="line">38</span><br><span class="line">39</span><br><span class="line">40</span><br><span class="line">41</span><br><span class="line">42</span><br><span class="line">43</span><br><span class="line">44</span><br></pre></td><td class="code"><pre><code class="hljs r">require<span class="hljs-punctuation">(</span>reshape2<span class="hljs-punctuation">)</span><br>require<span class="hljs-punctuation">(</span>circlize<span class="hljs-punctuation">)</span><br>f_CC_nV_o <span class="hljs-operator"><-</span> <span class="hljs-keyword">function</span><span class="hljs-punctuation">(</span>cellchat<span class="hljs-punctuation">,</span> measure<span class="hljs-operator">=</span><span class="hljs-string">'count'</span><span class="hljs-punctuation">,</span> LC<span class="hljs-operator">=</span><span class="hljs-string">"HSPC"</span><span class="hljs-punctuation">,</span> RC<span class="hljs-operator">=</span><span class="hljs-string">"CRPC"</span><span class="hljs-punctuation">,</span> use.Pos <span class="hljs-operator">=</span> <span class="hljs-built_in">T</span><span class="hljs-punctuation">,</span> use.mat <span class="hljs-operator">=</span> <span class="hljs-built_in">F</span><span class="hljs-punctuation">,</span> lc_filter<span class="hljs-operator">=</span><span class="hljs-literal">NULL</span><span class="hljs-punctuation">)</span><span class="hljs-punctuation">{</span><br> mat <span class="hljs-operator"><-</span> cellchat<span class="hljs-operator">@</span>net<span class="hljs-punctuation">[[</span>RC<span class="hljs-punctuation">]</span><span class="hljs-punctuation">]</span><span class="hljs-punctuation">[[</span>measure<span class="hljs-punctuation">]</span><span class="hljs-punctuation">]</span> <span class="hljs-operator">-</span> cellchat<span class="hljs-operator">@</span>net<span class="hljs-punctuation">[[</span>LC<span class="hljs-punctuation">]</span><span class="hljs-punctuation">]</span><span class="hljs-punctuation">[[</span>measure<span class="hljs-punctuation">]</span><span class="hljs-punctuation">]</span><br> <span class="hljs-keyword">if</span><span class="hljs-punctuation">(</span><span class="hljs-operator">!</span><span class="hljs-built_in">is.null</span><span class="hljs-punctuation">(</span>lc_filter<span class="hljs-punctuation">)</span><span class="hljs-punctuation">)</span><span class="hljs-punctuation">{</span><br> mat <span class="hljs-operator"><-</span> mat<span class="hljs-punctuation">[</span>lc_filter<span class="hljs-punctuation">,</span> lc_filter<span class="hljs-punctuation">]</span><br> <span class="hljs-punctuation">}</span><br> <span class="hljs-keyword">if</span><span class="hljs-punctuation">(</span>use.Pos<span class="hljs-punctuation">)</span><span class="hljs-punctuation">{</span><br> mat<span class="hljs-punctuation">[</span>mat <span class="hljs-operator"><</span> <span class="hljs-number">0</span><span class="hljs-punctuation">]</span> <span class="hljs-operator">=</span> <span class="hljs-number">0</span><br> <span class="hljs-punctuation">}</span><span class="hljs-keyword">else</span><span class="hljs-punctuation">{</span><br> mat<span class="hljs-punctuation">[</span>mat <span class="hljs-operator">></span> <span class="hljs-number">0</span><span class="hljs-punctuation">]</span> <span class="hljs-operator">=</span> <span class="hljs-number">0</span><br> mat <span class="hljs-operator"><-</span> <span class="hljs-operator">-</span>mat<br> <span class="hljs-punctuation">}</span><br> <span class="hljs-keyword">if</span><span class="hljs-punctuation">(</span><span class="hljs-operator">!</span>use.mat<span class="hljs-punctuation">)</span><span class="hljs-punctuation">{</span><br> mat <span class="hljs-operator"><-</span> melt<span class="hljs-punctuation">(</span>mat<span class="hljs-punctuation">)</span><br> mat <span class="hljs-operator"><-</span> mat<span class="hljs-punctuation">[</span>mat<span class="hljs-operator">$</span>value <span class="hljs-operator">></span> <span class="hljs-number">0</span><span class="hljs-punctuation">,</span> <span class="hljs-punctuation">]</span><br> colnames<span class="hljs-punctuation">(</span>mat<span class="hljs-punctuation">)</span> <span class="hljs-operator"><-</span> <span class="hljs-built_in">c</span><span class="hljs-punctuation">(</span><span class="hljs-string">'from'</span><span class="hljs-punctuation">,</span> <span class="hljs-string">'to'</span><span class="hljs-punctuation">,</span> <span class="hljs-string">'value'</span><span class="hljs-punctuation">)</span><br> <span class="hljs-punctuation">}</span><br> mat<br><span class="hljs-punctuation">}</span><br>f_CC_nV_sp <span class="hljs-operator"><-</span> <span class="hljs-keyword">function</span><span class="hljs-punctuation">(</span>mat<span class="hljs-punctuation">,</span> Nuse<span class="hljs-punctuation">,</span> is.source<span class="hljs-operator">=</span><span class="hljs-built_in">T</span><span class="hljs-punctuation">,</span> track.height<span class="hljs-operator">=</span><span class="hljs-number">0.3</span><span class="hljs-punctuation">,</span> ...<span class="hljs-punctuation">)</span><span class="hljs-punctuation">{</span><br> <span class="hljs-keyword">if</span><span class="hljs-punctuation">(</span>is.source<span class="hljs-punctuation">)</span><span class="hljs-punctuation">{</span><br> mat <span class="hljs-operator"><-</span> mat<span class="hljs-punctuation">[</span>mat<span class="hljs-operator">$</span>from <span class="hljs-operator">%in%</span> Nuse<span class="hljs-punctuation">,</span><span class="hljs-punctuation">]</span><br> idx <span class="hljs-operator"><-</span> order<span class="hljs-punctuation">(</span>mat<span class="hljs-operator">$</span>value<span class="hljs-punctuation">)</span><br> mat<span class="hljs-operator">$</span>to <span class="hljs-operator"><-</span> <span class="hljs-built_in">as.character</span><span class="hljs-punctuation">(</span>mat<span class="hljs-operator">$</span>to<span class="hljs-punctuation">)</span><br> mat <span class="hljs-operator"><-</span> mat<span class="hljs-punctuation">[</span>idx<span class="hljs-punctuation">,</span><span class="hljs-punctuation">]</span><br> mat<span class="hljs-operator">$</span>to <span class="hljs-operator"><-</span> factor<span class="hljs-punctuation">(</span>mat<span class="hljs-operator">$</span>to<span class="hljs-punctuation">)</span><br> <span class="hljs-punctuation">}</span><span class="hljs-keyword">else</span><span class="hljs-punctuation">{</span><br> mat <span class="hljs-operator"><-</span> mat<span class="hljs-punctuation">[</span>mat<span class="hljs-operator">$</span>to <span class="hljs-operator">%in%</span> Nuse<span class="hljs-punctuation">,</span><span class="hljs-punctuation">]</span><br> idx <span class="hljs-operator"><-</span> order<span class="hljs-punctuation">(</span>mat<span class="hljs-operator">$</span>value<span class="hljs-punctuation">)</span><br> mat<span class="hljs-operator">$</span>from <span class="hljs-operator"><-</span> <span class="hljs-built_in">as.character</span><span class="hljs-punctuation">(</span>mat<span class="hljs-operator">$</span>from<span class="hljs-punctuation">)</span><br> mat <span class="hljs-operator"><-</span> mat<span class="hljs-punctuation">[</span>idx<span class="hljs-punctuation">,</span><span class="hljs-punctuation">]</span><br> mat<span class="hljs-operator">$</span>from <span class="hljs-operator"><-</span> factor<span class="hljs-punctuation">(</span>mat<span class="hljs-operator">$</span>from<span class="hljs-punctuation">)</span><br> <span class="hljs-punctuation">}</span><br> chordDiagram<span class="hljs-punctuation">(</span>mat<span class="hljs-punctuation">,</span> annotationTrack <span class="hljs-operator">=</span> <span class="hljs-built_in">c</span><span class="hljs-punctuation">(</span><span class="hljs-string">"grid"</span><span class="hljs-punctuation">,</span> <span class="hljs-string">"axis"</span><span class="hljs-punctuation">)</span><span class="hljs-punctuation">,</span> preAllocateTracks <span class="hljs-operator">=</span> <span class="hljs-number">1</span><span class="hljs-punctuation">,</span>...<span class="hljs-punctuation">)</span><br> circos.trackPlotRegion<span class="hljs-punctuation">(</span>track.height<span class="hljs-operator">=</span>track.height<span class="hljs-punctuation">,</span> ylim <span class="hljs-operator">=</span> <span class="hljs-built_in">c</span><span class="hljs-punctuation">(</span><span class="hljs-number">0</span><span class="hljs-punctuation">,</span> <span class="hljs-number">1</span><span class="hljs-punctuation">)</span><span class="hljs-punctuation">,</span> panel.fun <span class="hljs-operator">=</span> <span class="hljs-keyword">function</span><span class="hljs-punctuation">(</span>x<span class="hljs-punctuation">,</span> y<span class="hljs-punctuation">)</span> <span class="hljs-punctuation">{</span><br> xlim <span class="hljs-operator">=</span> get.cell.meta.data<span class="hljs-punctuation">(</span><span class="hljs-string">"xlim"</span><span class="hljs-punctuation">)</span><br> ylim <span class="hljs-operator">=</span> get.cell.meta.data<span class="hljs-punctuation">(</span><span class="hljs-string">"ylim"</span><span class="hljs-punctuation">)</span><br> sector.name <span class="hljs-operator">=</span> get.cell.meta.data<span class="hljs-punctuation">(</span><span class="hljs-string">"sector.index"</span><span class="hljs-punctuation">)</span><br> xplot <span class="hljs-operator">=</span> get.cell.meta.data<span class="hljs-punctuation">(</span><span class="hljs-string">"xplot"</span><span class="hljs-punctuation">)</span><br> circos.text<span class="hljs-punctuation">(</span>mean<span class="hljs-punctuation">(</span>xlim<span class="hljs-punctuation">)</span><span class="hljs-punctuation">,</span> <span class="hljs-number">0.5</span><span class="hljs-punctuation">,</span> sector.name<span class="hljs-punctuation">,</span> facing <span class="hljs-operator">=</span> <span class="hljs-string">"clockwise"</span><span class="hljs-punctuation">,</span> niceFacing <span class="hljs-operator">=</span> <span class="hljs-built_in">T</span><span class="hljs-punctuation">,</span> track.index <span class="hljs-operator">=</span> <span class="hljs-number">3</span><span class="hljs-punctuation">)</span><br> <span class="hljs-punctuation">}</span><span class="hljs-punctuation">,</span> bg.border <span class="hljs-operator">=</span> <span class="hljs-literal">NA</span><span class="hljs-punctuation">)</span><br> circos.clear<span class="hljs-punctuation">(</span><span class="hljs-punctuation">)</span><br><span class="hljs-punctuation">}</span><br></code></pre></td></tr></table></figure>
- <figure class="highlight r"><table><tr><td class="gutter"><pre><span class="line">1</span><br><span class="line">2</span><br><span class="line">3</span><br><span class="line">4</span><br><span class="line">5</span><br><span class="line">6</span><br><span class="line">7</span><br><span class="line">8</span><br><span class="line">9</span><br><span class="line">10</span><br><span class="line">11</span><br><span class="line">12</span><br><span class="line">13</span><br><span class="line">14</span><br><span class="line">15</span><br><span class="line">16</span><br><span class="line">17</span><br><span class="line">18</span><br><span class="line">19</span><br></pre></td><td class="code"><pre><code class="hljs r">pdf<span class="hljs-punctuation">(</span>file <span class="hljs-operator">=</span> <span class="hljs-string">'nV_up_number_of_interactions_fF.pdf'</span><span class="hljs-punctuation">,</span> height <span class="hljs-operator">=</span> <span class="hljs-number">48</span><span class="hljs-punctuation">,</span> width <span class="hljs-operator">=</span> <span class="hljs-number">24</span><span class="hljs-punctuation">)</span><br>par<span class="hljs-punctuation">(</span>mfrow <span class="hljs-operator">=</span> <span class="hljs-built_in">c</span><span class="hljs-punctuation">(</span><span class="hljs-number">3</span><span class="hljs-punctuation">,</span><span class="hljs-number">2</span><span class="hljs-punctuation">)</span><span class="hljs-punctuation">,</span> xpd<span class="hljs-operator">=</span><span class="hljs-literal">TRUE</span><span class="hljs-punctuation">)</span><br>f_CC_nV_sp<span class="hljs-punctuation">(</span>f_CC_nV_o<span class="hljs-punctuation">(</span>SS<span class="hljs-punctuation">,</span> lc_filter <span class="hljs-operator">=</span> flt<span class="hljs-punctuation">)</span><span class="hljs-punctuation">,</span> <span class="hljs-string">'Fibroblasts'</span><span class="hljs-punctuation">)</span><br>f_CC_nV_sp<span class="hljs-punctuation">(</span>f_CC_nV_o<span class="hljs-punctuation">(</span>SS<span class="hljs-punctuation">,</span> measure <span class="hljs-operator">=</span> <span class="hljs-string">"weight"</span><span class="hljs-punctuation">,</span> lc_filter <span class="hljs-operator">=</span> flt<span class="hljs-punctuation">)</span><span class="hljs-punctuation">,</span> <span class="hljs-string">'Fibroblasts'</span><span class="hljs-punctuation">)</span><br>f_CC_nV_sp<span class="hljs-punctuation">(</span>f_CC_nV_o<span class="hljs-punctuation">(</span>ER<span class="hljs-punctuation">,</span> lc_filter <span class="hljs-operator">=</span> flt<span class="hljs-punctuation">)</span><span class="hljs-punctuation">,</span> <span class="hljs-string">'Fibroblasts'</span><span class="hljs-punctuation">)</span><br>f_CC_nV_sp<span class="hljs-punctuation">(</span>f_CC_nV_o<span class="hljs-punctuation">(</span>ER<span class="hljs-punctuation">,</span> measure <span class="hljs-operator">=</span> <span class="hljs-string">"weight"</span><span class="hljs-punctuation">,</span> lc_filter <span class="hljs-operator">=</span> flt<span class="hljs-punctuation">)</span><span class="hljs-punctuation">,</span> <span class="hljs-string">'Fibroblasts'</span><span class="hljs-punctuation">)</span><br>f_CC_nV_sp<span class="hljs-punctuation">(</span>f_CC_nV_o<span class="hljs-punctuation">(</span>CC<span class="hljs-punctuation">,</span> lc_filter <span class="hljs-operator">=</span> flt<span class="hljs-punctuation">)</span><span class="hljs-punctuation">,</span> <span class="hljs-string">'Fibroblasts'</span><span class="hljs-punctuation">)</span><br>f_CC_nV_sp<span class="hljs-punctuation">(</span>f_CC_nV_o<span class="hljs-punctuation">(</span>CC<span class="hljs-punctuation">,</span> measure <span class="hljs-operator">=</span> <span class="hljs-string">"weight"</span><span class="hljs-punctuation">,</span> lc_filter <span class="hljs-operator">=</span> flt<span class="hljs-punctuation">)</span><span class="hljs-punctuation">,</span> <span class="hljs-string">'Fibroblasts'</span><span class="hljs-punctuation">)</span><br>dev.off<span class="hljs-punctuation">(</span><span class="hljs-punctuation">)</span><br><br>pdf<span class="hljs-punctuation">(</span>file <span class="hljs-operator">=</span> <span class="hljs-string">'nV_up_number_of_interactions_tF.pdf'</span><span class="hljs-punctuation">,</span> height <span class="hljs-operator">=</span> <span class="hljs-number">48</span><span class="hljs-punctuation">,</span> width <span class="hljs-operator">=</span> <span class="hljs-number">24</span><span class="hljs-punctuation">)</span><br>par<span class="hljs-punctuation">(</span>mfrow <span class="hljs-operator">=</span> <span class="hljs-built_in">c</span><span class="hljs-punctuation">(</span><span class="hljs-number">3</span><span class="hljs-punctuation">,</span><span class="hljs-number">2</span><span class="hljs-punctuation">)</span><span class="hljs-punctuation">,</span> xpd<span class="hljs-operator">=</span><span class="hljs-literal">TRUE</span><span class="hljs-punctuation">)</span><br>f_CC_nV_sp<span class="hljs-punctuation">(</span>f_CC_nV_o<span class="hljs-punctuation">(</span>SS<span class="hljs-punctuation">,</span> lc_filter <span class="hljs-operator">=</span> flt<span class="hljs-punctuation">)</span><span class="hljs-punctuation">,</span> <span class="hljs-string">'Fibroblasts'</span><span class="hljs-punctuation">,</span> is.source <span class="hljs-operator">=</span> <span class="hljs-built_in">F</span><span class="hljs-punctuation">)</span><br>f_CC_nV_sp<span class="hljs-punctuation">(</span>f_CC_nV_o<span class="hljs-punctuation">(</span>SS<span class="hljs-punctuation">,</span> measure <span class="hljs-operator">=</span> <span class="hljs-string">"weight"</span><span class="hljs-punctuation">,</span> lc_filter <span class="hljs-operator">=</span> flt<span class="hljs-punctuation">)</span><span class="hljs-punctuation">,</span> <span class="hljs-string">'Fibroblasts'</span><span class="hljs-punctuation">,</span> is.source <span class="hljs-operator">=</span> <span class="hljs-built_in">F</span><span class="hljs-punctuation">)</span><br>f_CC_nV_sp<span class="hljs-punctuation">(</span>f_CC_nV_o<span class="hljs-punctuation">(</span>ER<span class="hljs-punctuation">,</span> lc_filter <span class="hljs-operator">=</span> flt<span class="hljs-punctuation">)</span><span class="hljs-punctuation">,</span> <span class="hljs-string">'Fibroblasts'</span><span class="hljs-punctuation">,</span> is.source <span class="hljs-operator">=</span> <span class="hljs-built_in">F</span><span class="hljs-punctuation">)</span><br>f_CC_nV_sp<span class="hljs-punctuation">(</span>f_CC_nV_o<span class="hljs-punctuation">(</span>ER<span class="hljs-punctuation">,</span> measure <span class="hljs-operator">=</span> <span class="hljs-string">"weight"</span><span class="hljs-punctuation">,</span> lc_filter <span class="hljs-operator">=</span> flt<span class="hljs-punctuation">)</span><span class="hljs-punctuation">,</span> <span class="hljs-string">'Fibroblasts'</span><span class="hljs-punctuation">,</span> is.source <span class="hljs-operator">=</span> <span class="hljs-built_in">F</span><span class="hljs-punctuation">)</span><br>f_CC_nV_sp<span class="hljs-punctuation">(</span>f_CC_nV_o<span class="hljs-punctuation">(</span>CC<span class="hljs-punctuation">,</span> lc_filter <span class="hljs-operator">=</span> flt<span class="hljs-punctuation">)</span><span class="hljs-punctuation">,</span> <span class="hljs-string">'Fibroblasts'</span><span class="hljs-punctuation">,</span> is.source <span class="hljs-operator">=</span> <span class="hljs-built_in">F</span><span class="hljs-punctuation">)</span><br>f_CC_nV_sp<span class="hljs-punctuation">(</span>f_CC_nV_o<span class="hljs-punctuation">(</span>CC<span class="hljs-punctuation">,</span> measure <span class="hljs-operator">=</span> <span class="hljs-string">"weight"</span><span class="hljs-punctuation">,</span> lc_filter <span class="hljs-operator">=</span> flt<span class="hljs-punctuation">)</span><span class="hljs-punctuation">,</span> <span class="hljs-string">'Fibroblasts'</span><span class="hljs-punctuation">,</span> is.source <span class="hljs-operator">=</span> <span class="hljs-built_in">F</span><span class="hljs-punctuation">)</span><br>dev.off<span class="hljs-punctuation">(</span><span class="hljs-punctuation">)</span><br></code></pre></td></tr></table></figure>
- <h2 id="饼图"><a href="#饼图" class="headerlink" title="饼图"></a>饼图</h2><figure class="highlight r"><table><tr><td class="gutter"><pre><span class="line">1</span><br><span class="line">2</span><br><span class="line">3</span><br><span class="line">4</span><br><span class="line">5</span><br><span class="line">6</span><br><span class="line">7</span><br><span class="line">8</span><br><span class="line">9</span><br><span class="line">10</span><br><span class="line">11</span><br><span class="line">12</span><br><span class="line">13</span><br><span class="line">14</span><br><span class="line">15</span><br><span class="line">16</span><br><span class="line">17</span><br><span class="line">18</span><br><span class="line">19</span><br><span class="line">20</span><br><span class="line">21</span><br><span class="line">22</span><br><span class="line">23</span><br><span class="line">24</span><br><span class="line">25</span><br><span class="line">26</span><br><span class="line">27</span><br><span class="line">28</span><br><span class="line">29</span><br><span class="line">30</span><br><span class="line">31</span><br><span class="line">32</span><br><span class="line">33</span><br><span class="line">34</span><br><span class="line">35</span><br><span class="line">36</span><br><span class="line">37</span><br><span class="line">38</span><br><span class="line">39</span><br><span class="line">40</span><br><span class="line">41</span><br><span class="line">42</span><br><span class="line">43</span><br><span class="line">44</span><br><span class="line">45</span><br><span class="line">46</span><br><span class="line">47</span><br><span class="line">48</span><br><span class="line">49</span><br></pre></td><td class="code"><pre><code class="hljs r">library<span class="hljs-punctuation">(</span>RColorBrewer<span class="hljs-punctuation">)</span><br>library<span class="hljs-punctuation">(</span>ggplot2<span class="hljs-punctuation">)</span><br>library<span class="hljs-punctuation">(</span>ggrepel<span class="hljs-punctuation">)</span><br>library<span class="hljs-punctuation">(</span>plyr<span class="hljs-punctuation">)</span><br>library<span class="hljs-punctuation">(</span>dplyr<span class="hljs-punctuation">)</span><br>library<span class="hljs-punctuation">(</span>patchwork<span class="hljs-punctuation">)</span><br>library<span class="hljs-punctuation">(</span>purrr<span class="hljs-punctuation">)</span><br>blank_theme <span class="hljs-operator"><-</span> theme_minimal<span class="hljs-punctuation">(</span><span class="hljs-punctuation">)</span><span class="hljs-operator">+</span><br> theme<span class="hljs-punctuation">(</span><br> axis.title.x <span class="hljs-operator">=</span> element_blank<span class="hljs-punctuation">(</span><span class="hljs-punctuation">)</span><span class="hljs-punctuation">,</span><br> axis.text.x<span class="hljs-operator">=</span>element_blank<span class="hljs-punctuation">(</span><span class="hljs-punctuation">)</span><span class="hljs-punctuation">,</span><br> axis.title.y <span class="hljs-operator">=</span> element_blank<span class="hljs-punctuation">(</span><span class="hljs-punctuation">)</span><span class="hljs-punctuation">,</span><br> axis.text.y<span class="hljs-operator">=</span>element_blank<span class="hljs-punctuation">(</span><span class="hljs-punctuation">)</span><span class="hljs-punctuation">,</span><br> panel.border <span class="hljs-operator">=</span> element_blank<span class="hljs-punctuation">(</span><span class="hljs-punctuation">)</span><span class="hljs-punctuation">,</span><br> panel.grid<span class="hljs-operator">=</span>element_blank<span class="hljs-punctuation">(</span><span class="hljs-punctuation">)</span><span class="hljs-punctuation">,</span><br> axis.ticks <span class="hljs-operator">=</span> element_blank<span class="hljs-punctuation">(</span><span class="hljs-punctuation">)</span><span class="hljs-punctuation">,</span><br> plot.title<span class="hljs-operator">=</span>element_text<span class="hljs-punctuation">(</span>size<span class="hljs-operator">=</span><span class="hljs-number">14</span><span class="hljs-punctuation">,</span> face<span class="hljs-operator">=</span><span class="hljs-string">"bold"</span><span class="hljs-punctuation">,</span>hjust <span class="hljs-operator">=</span> <span class="hljs-number">0.5</span><span class="hljs-punctuation">)</span><br> <span class="hljs-punctuation">)</span><br>col_Paired <span class="hljs-operator"><-</span> colorRampPalette<span class="hljs-punctuation">(</span>brewer.pal<span class="hljs-punctuation">(</span><span class="hljs-number">12</span><span class="hljs-punctuation">,</span> <span class="hljs-string">"Paired"</span><span class="hljs-punctuation">)</span><span class="hljs-punctuation">)</span><br>f_pie <span class="hljs-operator"><-</span> <span class="hljs-keyword">function</span><span class="hljs-punctuation">(</span>lc_x<span class="hljs-punctuation">,</span> lc_main<span class="hljs-punctuation">,</span> lc_x_p <span class="hljs-operator">=</span> <span class="hljs-number">1.3</span><span class="hljs-punctuation">,</span> lc_r <span class="hljs-operator">=</span> <span class="hljs-built_in">T</span><span class="hljs-punctuation">)</span><span class="hljs-punctuation">{</span><br> lc_cols <span class="hljs-operator"><-</span> col_Paired<span class="hljs-punctuation">(</span><span class="hljs-built_in">length</span><span class="hljs-punctuation">(</span>lc_x<span class="hljs-punctuation">)</span><span class="hljs-punctuation">)</span><br> lc_v <span class="hljs-operator"><-</span> as.vector<span class="hljs-punctuation">(</span><span class="hljs-number">100</span><span class="hljs-operator">*</span>lc_x<span class="hljs-punctuation">)</span><br> lc_df <span class="hljs-operator"><-</span> data.frame<span class="hljs-punctuation">(</span>type <span class="hljs-operator">=</span> <span class="hljs-built_in">names</span><span class="hljs-punctuation">(</span>lc_x<span class="hljs-punctuation">)</span><span class="hljs-punctuation">,</span> nums <span class="hljs-operator">=</span> lc_v<span class="hljs-punctuation">)</span><br> lc_df <span class="hljs-operator"><-</span> lc_df<span class="hljs-punctuation">[</span>order<span class="hljs-punctuation">(</span>lc_df<span class="hljs-operator">$</span>type<span class="hljs-punctuation">)</span><span class="hljs-punctuation">,</span><span class="hljs-punctuation">]</span><br> lc_percent <span class="hljs-operator">=</span> sprintf<span class="hljs-punctuation">(</span><span class="hljs-string">'%0.2f%%'</span><span class="hljs-punctuation">,</span>lc_df<span class="hljs-operator">$</span>nums<span class="hljs-punctuation">)</span><br> <span class="hljs-keyword">if</span><span class="hljs-punctuation">(</span>lc_r<span class="hljs-punctuation">)</span><span class="hljs-punctuation">{</span><br> lc_df<span class="hljs-operator">$</span>pos <span class="hljs-operator"><-</span> with<span class="hljs-punctuation">(</span>lc_df<span class="hljs-punctuation">,</span> <span class="hljs-number">100</span><span class="hljs-operator">-</span><span class="hljs-built_in">cumsum</span><span class="hljs-punctuation">(</span>nums<span class="hljs-punctuation">)</span><span class="hljs-operator">+</span>nums<span class="hljs-operator">/</span><span class="hljs-number">2</span><span class="hljs-punctuation">)</span><br> <span class="hljs-punctuation">}</span><span class="hljs-keyword">else</span><span class="hljs-punctuation">{</span><br> lc_df<span class="hljs-operator">$</span>pos <span class="hljs-operator"><-</span> with<span class="hljs-punctuation">(</span>lc_df<span class="hljs-punctuation">,</span> <span class="hljs-built_in">cumsum</span><span class="hljs-punctuation">(</span>nums<span class="hljs-punctuation">)</span><span class="hljs-operator">-</span>nums<span class="hljs-operator">/</span><span class="hljs-number">2</span><span class="hljs-punctuation">)</span><br> <span class="hljs-punctuation">}</span> <br> lc_pie <span class="hljs-operator"><-</span> ggplot<span class="hljs-punctuation">(</span>data <span class="hljs-operator">=</span> lc_df<span class="hljs-punctuation">,</span> mapping <span class="hljs-operator">=</span> aes<span class="hljs-punctuation">(</span>x <span class="hljs-operator">=</span> <span class="hljs-number">1</span><span class="hljs-punctuation">,</span> y <span class="hljs-operator">=</span> nums<span class="hljs-punctuation">,</span> fill <span class="hljs-operator">=</span> type<span class="hljs-punctuation">)</span><span class="hljs-punctuation">)</span> <span class="hljs-operator">+</span> geom_bar<span class="hljs-punctuation">(</span>stat <span class="hljs-operator">=</span> <span class="hljs-string">'identity'</span><span class="hljs-punctuation">)</span><br><span class="hljs-comment"># print(lc_df)</span><br><span class="hljs-comment"># print(lc_pie)</span><br> lc_pie <span class="hljs-operator"><-</span> lc_pie <span class="hljs-operator">+</span> coord_polar<span class="hljs-punctuation">(</span><span class="hljs-string">"y"</span><span class="hljs-punctuation">,</span> start<span class="hljs-operator">=</span><span class="hljs-number">0</span><span class="hljs-punctuation">,</span> direction <span class="hljs-operator">=</span> <span class="hljs-number">1</span><span class="hljs-punctuation">)</span> <span class="hljs-operator">+</span> scale_fill_manual<span class="hljs-punctuation">(</span>values<span class="hljs-operator">=</span>lc_cols<span class="hljs-punctuation">)</span> <span class="hljs-operator">+</span> blank_theme <br> lc_pie <span class="hljs-operator"><-</span> lc_pie <span class="hljs-operator">+</span> geom_text_repel<span class="hljs-punctuation">(</span>aes<span class="hljs-punctuation">(</span>x <span class="hljs-operator">=</span> lc_x_p<span class="hljs-punctuation">,</span> y<span class="hljs-operator">=</span>pos<span class="hljs-punctuation">)</span><span class="hljs-punctuation">,</span>label<span class="hljs-operator">=</span> lc_percent<span class="hljs-punctuation">,</span> force <span class="hljs-operator">=</span> <span class="hljs-built_in">T</span><span class="hljs-punctuation">,</span> <br> arrow <span class="hljs-operator">=</span> arrow<span class="hljs-punctuation">(</span><span class="hljs-built_in">length</span><span class="hljs-operator">=</span>unit<span class="hljs-punctuation">(</span><span class="hljs-number">0.01</span><span class="hljs-punctuation">,</span> <span class="hljs-string">"npc"</span><span class="hljs-punctuation">)</span><span class="hljs-punctuation">)</span><span class="hljs-punctuation">,</span> segment.color <span class="hljs-operator">=</span> <span class="hljs-string">"#cccccc"</span><span class="hljs-punctuation">,</span> segment.size <span class="hljs-operator">=</span> <span class="hljs-number">0.5</span><span class="hljs-punctuation">)</span><br> lc_pie <span class="hljs-operator"><-</span> lc_pie <span class="hljs-operator">+</span> labs<span class="hljs-punctuation">(</span>title <span class="hljs-operator">=</span> lc_main<span class="hljs-punctuation">)</span><br> lc_pie<br><span class="hljs-punctuation">}</span><br> <br>f_pie_metaN <span class="hljs-operator"><-</span> <span class="hljs-keyword">function</span><span class="hljs-punctuation">(</span>sObject<span class="hljs-punctuation">,</span> lc_group.by<span class="hljs-operator">=</span><span class="hljs-literal">NULL</span><span class="hljs-punctuation">)</span><span class="hljs-punctuation">{</span><br> <span class="hljs-keyword">if</span><span class="hljs-punctuation">(</span><span class="hljs-operator">!</span><span class="hljs-built_in">is.null</span><span class="hljs-punctuation">(</span>lc_group.by<span class="hljs-punctuation">)</span><span class="hljs-punctuation">)</span><span class="hljs-punctuation">{</span><br> sObject <span class="hljs-operator">=</span> sObject<span class="hljs-punctuation">[[</span>lc_group.by<span class="hljs-punctuation">]</span><span class="hljs-punctuation">]</span><br> <span class="hljs-punctuation">}</span><span class="hljs-keyword">else</span><span class="hljs-punctuation">{</span><br> lc_group.by <span class="hljs-operator">=</span> colnames<span class="hljs-punctuation">(</span>sObject<span class="hljs-punctuation">)</span><br> <span class="hljs-punctuation">}</span><br> tp_data <span class="hljs-operator"><-</span> prop.table<span class="hljs-punctuation">(</span>table<span class="hljs-punctuation">(</span>sObject<span class="hljs-punctuation">)</span><span class="hljs-punctuation">)</span><br> f_pie<span class="hljs-punctuation">(</span>tp_data<span class="hljs-punctuation">,</span> sprintf<span class="hljs-punctuation">(</span><span class="hljs-string">'Proportion of %s'</span><span class="hljs-punctuation">,</span> lc_group.by<span class="hljs-punctuation">)</span><span class="hljs-punctuation">)</span><br><span class="hljs-punctuation">}</span><br></code></pre></td></tr></table></figure>
- <h2 id="点图"><a href="#点图" class="headerlink" title="点图"></a>点图</h2><figure class="highlight r"><table><tr><td class="gutter"><pre><span class="line">1</span><br><span class="line">2</span><br><span class="line">3</span><br><span class="line">4</span><br><span class="line">5</span><br></pre></td><td class="code"><pre><code class="hljs r">p <span class="hljs-operator"><-</span> DotPlot<span class="hljs-punctuation">(</span>sce<span class="hljs-punctuation">,</span> features <span class="hljs-operator">=</span> marker<span class="hljs-operator">$</span>marker<span class="hljs-punctuation">)</span><span class="hljs-operator">+</span>coord_flip<span class="hljs-punctuation">(</span><span class="hljs-punctuation">)</span><span class="hljs-operator">+</span>theme_bw<span class="hljs-punctuation">(</span><span class="hljs-punctuation">)</span> <br>p <span class="hljs-operator"><-</span> p <span class="hljs-operator">+</span> theme<span class="hljs-punctuation">(</span>panel.grid<span class="hljs-operator">=</span>element_blank<span class="hljs-punctuation">(</span><span class="hljs-punctuation">)</span><span class="hljs-punctuation">,</span> axis.text.x<span class="hljs-operator">=</span>element_text<span class="hljs-punctuation">(</span>angle<span class="hljs-operator">=</span> <span class="hljs-number">45</span><span class="hljs-punctuation">,</span>hjust <span class="hljs-operator">=</span> <span class="hljs-number">1</span><span class="hljs-punctuation">,</span>vjust<span class="hljs-operator">=</span> <span class="hljs-number">0.5</span><span class="hljs-punctuation">)</span><span class="hljs-punctuation">)</span><br>p <span class="hljs-operator"><-</span> p <span class="hljs-operator">+</span> scale_color_gradientn<span class="hljs-punctuation">(</span>values<span class="hljs-operator">=</span> seq<span class="hljs-punctuation">(</span><span class="hljs-number">0</span><span class="hljs-punctuation">,</span><span class="hljs-number">1</span><span class="hljs-punctuation">,</span><span class="hljs-number">0.2</span><span class="hljs-punctuation">)</span><span class="hljs-punctuation">,</span>colours <span class="hljs-operator">=</span> <span class="hljs-built_in">c</span><span class="hljs-punctuation">(</span> <span class="hljs-string">'#330066'</span><span class="hljs-punctuation">,</span> <span class="hljs-string">'#336699'</span><span class="hljs-punctuation">,</span> <span class="hljs-string">'#66CC66'</span><span class="hljs-punctuation">,</span> <span class="hljs-string">'#FFCC33'</span><span class="hljs-punctuation">)</span><span class="hljs-punctuation">)</span><br>p <span class="hljs-operator"><-</span> p <span class="hljs-operator">+</span> labs<span class="hljs-punctuation">(</span>x<span class="hljs-operator">=</span><span class="hljs-literal">NULL</span><span class="hljs-punctuation">,</span> y<span class="hljs-operator">=</span><span class="hljs-literal">NULL</span><span class="hljs-punctuation">)</span><br>p <span class="hljs-operator"><-</span> p <span class="hljs-operator">+</span>guides<span class="hljs-punctuation">(</span>size<span class="hljs-operator">=</span>guide_legend<span class="hljs-punctuation">(</span>order<span class="hljs-operator">=</span> <span class="hljs-number">3</span><span class="hljs-punctuation">)</span><span class="hljs-punctuation">)</span> <br></code></pre></td></tr></table></figure>
- <h2 id="UMAP图"><a href="#UMAP图" class="headerlink" title="UMAP图"></a>UMAP图</h2><figure class="highlight r"><table><tr><td class="gutter"><pre><span class="line">1</span><br><span class="line">2</span><br><span class="line">3</span><br><span class="line">4</span><br><span class="line">5</span><br><span class="line">6</span><br></pre></td><td class="code"><pre><code class="hljs r">library<span class="hljs-punctuation">(</span>Seurat<span class="hljs-punctuation">)</span><br>library<span class="hljs-punctuation">(</span>ggsci<span class="hljs-punctuation">)</span><br><span class="hljs-built_in">length</span><span class="hljs-punctuation">(</span>levels<span class="hljs-punctuation">(</span>sce<span class="hljs-operator">@</span>meta.data<span class="hljs-operator">$</span>cell_type_fig1spA<span class="hljs-punctuation">)</span><span class="hljs-punctuation">)</span><br>p <span class="hljs-operator"><-</span> DimPlot<span class="hljs-punctuation">(</span>sce<span class="hljs-punctuation">,</span> reduction <span class="hljs-operator">=</span> <span class="hljs-string">'umap'</span><span class="hljs-punctuation">,</span> group.by <span class="hljs-operator">=</span> <span class="hljs-string">'cell_type_fig1spA'</span><span class="hljs-punctuation">,</span> label <span class="hljs-operator">=</span> <span class="hljs-built_in">T</span><span class="hljs-punctuation">,</span> repel <span class="hljs-operator">=</span> <span class="hljs-built_in">T</span><span class="hljs-punctuation">,</span> cols <span class="hljs-operator">=</span> pal_d3<span class="hljs-punctuation">(</span><span class="hljs-string">"category20"</span><span class="hljs-punctuation">,</span> alpha <span class="hljs-operator">=</span> <span class="hljs-number">0.6</span><span class="hljs-punctuation">)</span><span class="hljs-punctuation">(</span><span class="hljs-number">19</span><span class="hljs-punctuation">)</span><span class="hljs-punctuation">,</span> pt.size <span class="hljs-operator">=</span> <span class="hljs-number">1</span><span class="hljs-punctuation">)</span><br>p<br>ggsave<span class="hljs-punctuation">(</span>p<span class="hljs-punctuation">,</span> filename <span class="hljs-operator">=</span> <span class="hljs-string">'fig1.SpA_12inch.pdf'</span><span class="hljs-punctuation">,</span> width <span class="hljs-operator">=</span> <span class="hljs-number">12</span><span class="hljs-punctuation">,</span> height <span class="hljs-operator">=</span> <span class="hljs-number">12</span><span class="hljs-punctuation">)</span><br></code></pre></td></tr></table></figure>
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